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Sains Malaysiana 54(3)(2025): 641-651
http://doi.org/10.17576/jsm-2025-5403-03
Effects of Eyestalk Ablation and Limb Autotomy on Moulting, Growth and
Survival Rate of Orange Mud Crab, Scylla olivacea Juveniles for the Production of Soft-Shell Crabs
(Kesan Pembuangan Tangkai Mata dan Autotomi Anggota Badan terhadap Proses Penyalinan Kulit, Pertumbuhan dan Kadar Kemandirian Juvenil Ketam Bakau, Scylla olivacea untuk Pengeluaran Ketam Kulit Lembut)
MHD
IKHWANUDDIN1,2,*, HANAFIAH FAZHAN1,2,3,4, YOUJI WANG5, ALEXANDER CHONG
SHU-CHIEN3,6, MUYASSAR H. ABUALREESH7, MOHAMAD JALILAH1,
AMBOK BOLONG ABOL-MUNAFI1 & KHOR WAIHO1,2,3,4
1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Terengganu,
Terengganu, Malaysia
Received: 4
October 2023/Accepted: 6 December 2024
Abstract
Soft-shell
crabs are highly sought after due to their ease of storage and can be eaten
whole, including the outer carapace. The high demand for soft-shell crabs leads
to the exploration of different production techniques and methods. This study
explores the effect of limb autotomy, eyestalk ablation and their combinations
on the duration and success rate till the next moulting, and the size increment
and survival rate of male juveniles of Scylla olivacea after moult. Additionally, female juveniles were also subjected to limb
autotomy to investigate the potential impact of sex on their response to the
procedure. Full limb autotomy (T2), either alone or with eyestalk ablation
(T4), induced the fastest moulting (average 33 d and 39 d, respectively),
followed by eyestalk ablation (average 57 d) and control (average 82 d). Limb
autotomy, either alone or with eyestalk ablation, resulted in 100% and 90%
moulting success, respectively, whereas that of eyestalk ablation and control
was 50% and 56.83%, respectively. Crabs subjected to both moult-inducing
techniques had the lowest size and weight gain after moulting. Females moulted
faster than males in natural conditions, but this was negligible when limb
autotomy was introduced. Limb autotomy had no impact on the CW increment and
survival rate of both sexes, but greatly reduced their BW increment. Thus, limb
autotomy can be incorporated in the production of soft-shell crabs to reduce
turnover time.
Keywords:
Eyestalk ablation; limb autotomy; mud crab; Scylla olivacea;
soft-shell crab
Abstrak
Ketam kulit lembut sangat digemari kerana kemudahan penyimpanannya dan boleh dimakan keseluruhan, termasuklah cangkerang luarnya. Permintaan yang tinggi untuk ketam kulit lembut membawa kepada penerokaan teknik dan kaedah pengeluarannya yang berbeza. Kajian ini meneroka kesan autotomi anggota badan, pembuangan tangkai mata dan gabungannya terhadap tempoh dan kadar kejayaan sehingga proses bersalin cangkerang seterusnya, serta peningkatan saiz dan kadar kemandirian juvenil jantan Scylla olivacea selepas bersalin cangkerang. Tambahan pula, ketam betina juvenil juga dikenakan autotomi anggota badan untuk mengkaji kemungkinan kesan jantina terhadap tindak balas mereka bagi prosedur tersebut. Autotomi anggota badan sepenuhnya (T2), sama ada secara bersendirian atau gabungan bersama dengan pembuangan tangkai mata (T4) mempercepatkan proses penyalinan cangkerang (purata masing-masing 33 hari dan 39 hari) diikuti oleh ablasi tangkai mata (purata 57 hari) dan kawalan (purata 82 hari). Autotomi anggota badan, sama ada secara bersendirian atau bersama ablasi tangkai mata, masing-masing menghasilkan kejayaan bersalin cangkerang 100% dan 90%, manakala ablasi tangkai mata dan kawalan masing-masing hanya mencapai 50% dan 56.83%. Ketam yang dikenakan kedua-dua teknik induksi penyalinan cangkerang mencatatkan peningkatan saiz dan berat badan yang paling rendah selepas bersalin cangkerang. Ketam betina bersalin cangkerang dengan lebih pantas berbanding jantan dalam keadaan semula jadi, tetapi perbezaan ini menjadi tidak ketara apabila autotomi anggota diperkenalkan. Autotomi anggota badan tidak memberikan kesan terhadap peningkatan lebar cangkerang (CW) dan kadar kemandirian hidup kedua-dua jantina, tetapi mengurangkan peningkatan berat badan (BW) secara ketara. Oleh itu, autotomi anggota badan boleh diamalkan dalam penghasilan ketam kulit lembut untuk memendekkan masa kitaran.
Kata kunci: Autotomi anggota badan; ketam bakau; ketam kulit lembut; pembuangan tangkai mata; Scylla olivacea
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